Animals and ethical approval Male Fischer 344 rats (8 weeks old, Charles River Laboratories Japan, Kanagawa, Japan) were acclimated for 4 weeks in the Laboratory Animal Research Center of the University of Occupational and Environmental Health, Japan, with free access to a standard commercial diet (manufacturer, catalog no.) and tap water under controlled environmental conditions (22 2 C, 55 10% humidity, 12 h light/dark cycle)
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Step-by-step resources for planning, ordering, and follow-up
Keep in original packaging away from light
Although many kits are available, most of these kits have substantial mark-up that is not always combined with quality
Use the same rotation schedule, the same technique, and the same tracking method for every subcutaneous injection in your regimen