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Slides were dehydrated, mounted using VectaMount Permanent mounting media (Vector Laboratories, H-5000-60) and imaged using 20 bright-field objective on a Zeiss AxioScan Z.1 slide scanner
Addition of free ligands inhibited the cellular internalization of targeted nanocarriers suggesting the crucial role of ligands in the uptake process
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Quality controls 99% HPLC purity, mass-spectrometry identity confirmation (BPC-157 expected mass ~1419.5 Da), and explicit endotoxin status are the four data points to verify per batch regardless of supplier
PMID 12729762
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