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overexpressed miR-340-5p in the myocardium by direct myocardial injection of AVV-9 containing miR-340-5p precursor, which targeted inhibition of MyD88 and thus restored declining GSH levels, alleviated insult of cardiac function and oxidative injury induced by sepsis in the hearts of mice [253]

Phase separation was induced by addition of ice-cold water (150 l), followed by vortexing, incubation at 4 C (10 min) and centrifugation (1,000 g , 10 min, 4 C)

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