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Patients utilizing this regenerative protocol frequently report that it: Accelerates the healing of muscles, tendons, and torn ligaments

Lipofectamine 3000 siRNA transfection reagent (Thermo Fisher Scientific, Carlsbad, CA, USA) was then used to transfect the cells with either negative control or Nrf2 siRNA duplexes at a concentration of 50 nM for 36 h

Storage is at 28C with a stability window of up to 60 days post-reconstitution

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The mobile phase for this method consists of water, acetonitrile (MeCN), and sulfuric acid, which serves as a buffer