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2.8 M), AMSH-LP (5 M), and Ub (2.4 mM) were mixed in elongation buffer [50 mM Tris-HCl (pH 9.0) containing ATP (10 mM), MgCl 2 (10 mM), and DTT (0.6 mM)], and incubated (30 C) (Fig
Ge P, Luo Y, Yang Q, et al
2025AHGXZK50099)
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