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Your solution is simply more dilute than intended
Gene silencing by small interfering RNA Transient gene silencing was attained by transfection of small interfering RNA (siRNA) into cells using siPORT lipid transfection reagent according to the manufacturers instructions
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Absence of Growth-Promoting and Diabetogenic Effects Full-length growth hormone can stimulate: Linear growth Organ enlargement Elevation of IGF-1 Reduced insulin sensitivity in some contexts However, research demonstrated that the 176191 fragment does not replicate these endocrine effects This distinction is critical to understanding why the fragment was investigated separately from full HGH
Slipczuk L, Bekinschtein P, Katche C, Cammarota M, Izquierdo I, Medina JH