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Cardiolipin externalization mediates prion protein (PrP) peptide 106-126-associated mitophagy and mitochondrial dysfunction
SKT, RS and JK provided technical suggestions and reviewed the manuscript
Ann Rheumatic Dis (2015) 74(Suppl 2):726
Researchers choose between the formulations according to the biological pathways they wish to investigate
Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached
Plates were incubated at 37C for 2 h and then fluorescence was measured with excitation at 360 nm and emission at 460 nm using a ClarioStar Monochromator Microplate Reader (BMG LABTECH, Ortenberg, Germany)
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