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Based on these conflicted results, the association was speculated to be related to the clinical features of the population, stages of the disease, severity of copper excess, and types of detected specimens in these studies

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Ramachandran plot analysis showed 88.7% of residues in the core region, 11.3% in the allowed region and no residues in the disallowed region

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To confirm the MTT results, a replicate plate run in parallel was developed by adding 5 l of 2.5 M Calcein-AM (ThermoFisher Scientific) to selected wells and incubated 15 min at room temperature