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Whole-cell extracts were collected and then centrifuged at 500 g for 5 min at 4 C, washed 3 with ice-cold 1 PBS and lysed using lysis buffer (8 M urea, 200 mM sodium bicarbonate and complete protease inhibitor cocktail (Roche, 000000011697498001))
The serious side effects of Ozempic include: Risk of thyroid tumors, including cancer: Ozempic has a boxed warning for the risk of thyroid tumors
Acknowledgments The publication fee for this work was covered by the Italian Ministry of Healths Ricerca Corrente funding to the IRCCS Humanitas Research Hospital
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