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The schedule continues upward7.5 mg, then 10 mg, then 12.5 mg, and finally 15 mg at maximumwith 4-week intervals between each step

Finally, we performed ligation and transformation, then selection and sequencing

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cDNA synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN) or High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to manufacturers instructions