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Briefly, pancreatic tissue was digested in Geys balanced salt solution containing 0.75 mg/mL collagenase P (Sigma-Aldrich), 0.03% protease (Roche, Mannheim, Germany), and 0.01% DNase I (Roche)

However, similar to the effects of fasting regimens, reduction in insulin sensitivity was observed in a short-term time frame so further studies are required for longer-term impact assessment

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Food Chem Toxicol 96:263272 Song WM, Colonna M (2018) The identity and function of microglia in neurodegeneration