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This creates a concentration of 5mg per mL, making dose calculations straightforward

2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

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10.1002/ijc.29535 106 HattoriA.TsunodaM.KonumaT.KobayashiM.NagyT.GlushkaJ.et al

Then dilute it further with 250 ml or 500 ml of saline or 5% glucose solution before giving it as a drip

We detected a robust increase in the expression of markers of JNK activation in the cyst cells of flies kept on an aripiprazole-supplemented diet (Fig