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13 C NMR (100 MHz, DMSO-d6) = 106.9, 100.6, 86.8, 86.0, 30.5, 22.0, 21.9, 18.4, 18.3
After 72 h treatments, 0.5 g ml 1 propidium iodide (Sigma-Aldrich, P4864) and 1.4 g ml 1 Hoechst 33342 (Invitrogen, H3570) were added to cells
For example, R49Q is a mutation located in the Gln-binding pocket of the N-terminal domain, and this mutation causes the loss of hydrogen bonds not only to the second -sheet but also to Gln
Cardiovascular disease is largely caused by oxidative stress in heart tissues
Schmidt C, Kisselbach J, Schweizer PA, et al
Furthermore, we note Y211C/H are variants associated with SPCDchanges that likely causes large structural perturbation of the carnitine-binding site beyond simply affecting solvent structure
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