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DSK, Japan) and transferred to a chamber containing oxygenated artificial cerebrospinal fluid (aCSF: 130 mM NaCl, 24 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 , 3.5 mM KCl, 1.5 mM CaCl 2 , 1.5 mM MgCl 2 , and 10 mM D(+)-glucose, pH 7.4)
Wuyts et al., 2018)
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To further verify that the catalytic activity of LOXL2 was responsible for the increase in BHZ signal, we used the following negative controls: (1) the overexpression of catalytically inactive H626/628Q LOXL2 double mutant (LOXL2-DM) 39,40 , (2) inhibition of LOXL2 specific activity using the small molecule inhibitor PAT-1251 (10 M) in cells overexpressing catalytically active LOXL2 41 , and (3) inhibition of total LOX activity using the non-selective small molecule LOX inhibitor -Aminopropionitrile (BAPN
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Frasch MG, Yoon BJ, Helbing DL et al (2023) Autism Spectrum Disorder: A Neuro-Immunometabolic Hypothesis of the Developmental Origins
