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In patients with severe liver disease, portal blood bypassing the liver leads to increased levels of ammonia and other toxic substances in the blood, which can lead to complications such as brain damage, sarcopenia and muscle spasticity [204]

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Anything less, and you risk increasing oxidative stress and decreasing natural glutathione levels

Briefly, whole-cell lysate was obtained by cell lysis buffer in the presence of a protease inhibitor cocktail (Sigma-Aldrich)