Results clearly showed that the tyrosine-nitration level was reduced in proportion to the molar ratios of GSSSG and LASSS relative to HGF, as revealed by the decrease in anti-nitroY mAb-positive HGF (60-kDa -chain and 90-kDa proform) normalized to total HGF -chain as a loading control, that was monitored by stripping with SDS-ME solution and re-probing the blot with anti-HGF -chain N-terminal domain mAb (Figure 1b,c, second and third rows)
DOI: 10.7759/cureus.73687
The anti-aging and recovery benefits are heavily tied to elevated GH/IGF-1 promoting cellular repair, collagen synthesis, and deep sleep
PMID: 34012345 Thompson, R., et al
10.1371/journal.pone.0007877 129
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