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After incubation, the filter spots were dried again before extracting the metabolites using 300 L of 5 mM ammonium acetate in methanol from the lower 96-well plate

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(Luzio et al., 2009

GHK-Cu (Copper Tripeptide) GHK-Cu is a naturally occurring copper-binding tripeptide studied extensively in dermal and extracellular matrix research

Clinics now use it off-label for adults with excess visceral fat, always under medical supervision