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Strong target identification normally requires orthogonal evidence such as direct binding, receptor-specific functional assays, selective antagonism, genetic loss of function, rescue experiments, and biophysical or chemical target-engagement analysis

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It is widely used in laboratory studies to investigate GHRH receptor signalling, growth hormone (GH) pathway dynamics, and associated hormonal cascades such as insulinlike growth factor1 (IGF1) activation all within controlled, nonclinical environments

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