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After the treatment period, the culture medium of each well was replaced by 0.2 ml of calcein AM solution (Sigma-Aldrich) at 2 M in phosphate buffer [PBS 1X] and the culture plates were put into the incubator for 30 minutes before fluorescence reading using a Synergy H1 microplate reader (Biotek, Winooski, VT, USA

Cytosolic and mitochondrial pools are functionally compartmentalized, and exchange between these domains occurs indirectly through metabolite shuttles [110]

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They already have that give it back to them at much higher pharmacologic doses and it brings their blood sugar down

This makes the experience easier to handle