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Quantification of the methylation of APC, GSTP1 and RASSF1A genes The extent of DNA methylation of three HCC-related host genes, APC, GSTP1 and RASSF1A , was determined by previously developed qMSP assays for APC 29 , GSTP1 31 and RASSF1A 48

Vitamin C (ascorbic acid) : Acts as a powerful antioxidant, protecting cells from damage by free radicals

Minimizing injection site reactions Proper injection technique matters enormously

do not expose to excessive heat

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Performing both meta-regression and subgroup analysis was not feasible in this context due to the restricted number of studies available