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After pooling the fractions containing pure 3,4- cis -leucocyanidin ( 6 ), MOPSO was added to a final concentration of 10 mM, the pH was set to 6.2 using 5 N NaOH and the pooled fractions were rotary-evaporated to a concentration of 7 mM of 3,4- cis -leucocyanidin ( 6 ), quantified by HPLC-UV (280 nm) with an external calibration curve with 3,4- trans -leucocyanidin ( 14 )

doi: 10.3389/fphar.2021.844785

233,234 In this process, toll-like receptors (TLRs) and NF-B play important roles

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