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Del Prato S, Kang J, Trautmann ME, Stewart J, Sorli CH, Derwahl M, et al

doi:10.1016/j.freeradbiomed.2010.08.011 Brown RC, Morris AP, ONeil RG (2007) Tight junction protein expression and barrier properties of immortalized mouse brain microvessel endothelial cells

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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

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