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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor
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70 This evolutionary dynamic has been demonstrated in NSCLC and melanoma treated with PD-1 blockade and explains why tumors with high mutational burden may still acquire rapid resistance to immunotherapy
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GLP-1 (Glucagon-like peptide-1) injections are a class of medications that mimic the action of the natural GLP-1 hormone in the body, stimulating insulin secretion, suppressing appetite, and delaying gastric emptying, thereby promoting weight loss
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