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These additional preparation stages introduce more handling time and increase workflow complexity
Holotoxin delivery and cell viability (CellTiter-Glo assay) 6His-tagged proteases fused to the BoNT translocation domain via a thrombin-cleavable linker were expressed and purified as described above
(PubMed) Fratantonio D, Speciale A, Molonia MS, et al
A., Horner, J
Uncover the nuances of well MB12, a highly bioavailable form of vitamin B12, as we explore its advantages and potential considerations in the realm of B12 supplementation
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