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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

The condition has been linked to obesity, type 2 diabetes, elevated cholesterol levels and high blood pressure, according to the Cleveland Clinic

Key References Burlina S, Dalfra MG, Caprino R, Lapolla A

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Counter frozen meals deliver 30g protein at 310-370 calories, the highest protein-to-calorie ratio in the freezer aisle