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The work is supported through funding from a major R01 grant from the National Institutes of Healths National Institute on Minority Health and Health Disparities

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Ghrelin is a hormone that increases plasma concentration before feeding and starts feeding, and it is known that ghrelin concentration rises immediately before feeding when restricted feeding is performed

Manufacturer instructions for pharmaceutical GH specify sterile water only

Before the substrate assay, DMEM was aspirated, and the cells were washed twice with 100 L of pre-warmed TB