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The mice were housed at a constant temperature and humidity under a 12-h/12-h lightdark cycle with free access to standard food and water for at least 1 week prior to the beginning of the experimental procedures

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Introducing DMSO concentrations higher than 50% into the blood resulted in instant hemolysis, white cell stacking and fibrinogen precipitation while direct injection of DMSO intravenously could cause local irritation and necrosis 6