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One of the most commonly used experimental models of IBD is the administration of an enema containing the contact-sensitizing allergen trinitrobenzenesulfonic acid (TNBS) in ethanol resulting in the development of macroscopic, histologic, and biochemical alterations that are well-characterized (Rachmilewitz et al, 1989
Those who have struggled with repeated diet attempts and weight regain are good candidates
Mice were treated for 12 weeks before being sacrificed
These attacks & their outcomes have been described in an article by Yanagisawa et al (2006)
Benzyl alcohol maintains sterility for multi-use access within this window
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