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During the manuscript submission process, two other fluorescent probes were independently reported to monitor GSH dynamics in living cells 38,39

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The presence of LOX immunoreactivity in these matrices was also investigated by immunofluorescence

GSH is a tripeptide (-L-glutamyl-L-cysteinyl-glycine) and is considered the predominant non-protein thiol in mammalian cells, working alone or in concert with enzymes to reduce superoxide radicals, hydroxyl radicals, and peroxynitrites.42 The intracellular balance and optimal concentration of GSH, as well as its redox ratio, are critical components of redox homeostasis in healthy cells.43 In this study, both melatonin and GSH administration were found to restore the disrupted levels of GSH-Px, SOD, and CAT in the CARB-treated group, supporting their role in maintaining antioxidant defense