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Repeated freeze-thaw cycles cause mechanical stress and can lead to compound aggregation or precipitation out of solution

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The organic layer was dried over anhydrous NaSO, filtered, and concentrated under reduced pressure to obtain Compound 2 (yield: 98%)

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Oxidized glutathione stimulates the formation of disulfide bonds between adjacent thiol groups in the protein molecule and changes their properties: GSSG + Pt-(SH)2 Pt(S-S) + 2GSH, and also interacts with SH-groups of proteins and forms "mixed disulfides", which changes the properties of proteins, causing dysfunction of cells