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Glutamate can be further converted to -ketoglutarate (-KG) through oxidative deamination by glutamate dehydrogenase (GLUD1) or transamination by glutamate-linked transaminase as an anaplerotic substrate in the TCA cycle for energy production (Figure 1)

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doi: 10.1021/acschemneuro.6b00056 3 AlexopoulosP.SorgC.FrschlerA.GrimmerT.SkokouM.WohlschlgerA.et al

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The mRNA expression of target genes was calculated by the 2 -Ct method with normalizing to 18 s mRNA expression

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