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Methods: Experiments were performed on adult (6 months) and old (24 months) Wistar rats divided into three groups: adult, old and glutathionetreated old rats
Nrf2 staining and translocation For visualization and quantification of endogenous Nrf2 translocation into the nucleus, glass 96-well plates were coated with fibronectin (1 g/ml) (Sigma) and poly-l-ornithine (40 g/ml) over night
Variants in GIPR, FTO, and MC4R genes correlate with differential response to peptide agonists
Compared to BPC-157, which acts primarily via the nitric oxide and growth hormone receptor pathways, and TB-500, which functions through actin sequestration and VEGF signalling, GHK-Cu operates largely through copper-mediated gene expression modulation and extracellular matrix remodelling
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