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Although in cognitively healthy individuals differences in BBB uptake have been observed with APOE genotypes as early as in individuals in their 30s (Yassine et al., 2017) in mice older ages may be required for such disturbances to occur (Alata et al., 2015)

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All collected samples were diluted to a protein concentration of 1 ng/l, and 5 l of each sample was loaded on a Tris-Glycine gel (Novex Tris-Glycine Mini Protein Gels, 1020%, 1.0 mm, WedgeWell) for Western blot analysis

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