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ELISA for FcRn binding analysis 96-well EIA/RIA Clear Flat Bottom Polystyrene Microplates (Corning) were coated overnight at 4 C with 8 g/mL (100 L/well) of a recombinant Fc-engineered human IgG1 mutant (YTE/KF) 98 , with specificity for 4-hydroxy-3-iodo-5-nitrophenylactic acid, diluted in DPBS (pH 7.4) (Sigma-Aldrich)
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