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TMT ratio check The combined and dried ratio check sample was re-dissolved in Buffer A (5% CH 3 CN, 95% water, 0.1% formic acid, 20 L) and desalted using C18 stage tips prepared in-house using 3 C18 discs (3 M Empore) stacked in 200 L pipette tips

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The resin was washed with binding buffer to wash away prays