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After 24 hours treatment, cells were washed and harvested in cold PBS and lysed in lysis buffer (25 mM TrisHCl, pH 7.5, 100 mM NaCl, 2.5 mM EDTA, 2.5 mM EGTA, 20 mM NaF, 1 mM Na3VO4, 20 mM sodium -glycerophosphate, 10 mM sodium pyrophosphate, 0.5% Triton X-100) containing protease inhibitor cocktail (Roche) and 50 mM iodoacetamide (IAM, Sigma-Aldrich)
Preclinical models suggest it supports blood vessel growth, reduces inflammation, and enhances tissue repair
The mechanism: hypothalamic GHSR-1a activation triggers CRH release pituitary corticotroph activation ACTH adrenal cortisol production
Molecular Microbiology, 68 (1), 416
complete recovery from mydriasis may require 24 hours in some patients
10.1001/archneur.57.10.1439 Arch
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