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To be used in conjunction with a program of reduced intake of dietary calories and increased physical activity
as well as improved mobility, sexual function, fertility, and quality of life
the structure is not
Lentiviruses were generated by co-transfection of each shRNA construct with envelope plus packaging vectors (pMD2.G, Addgene #12260 and psPAX2, Addgene #12259) and viral supernatants purified by ultracentrifugation onto 20% sucrose gradients
BDNF, brain-derived neurotrophic factor
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