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This is normal and does not indicate protocol failure

Mechanistically, drugs such as mecamylamine act as noncompetitive antagonists at neuronal nicotinic acetylcholine receptors, producing a poretrapping block by entering and becoming trapped within the open ion channel (second panel)

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Pharmacol Res 32:383389 Virmani MA, Caso V, Spadoni A, Rossi S, Russo F, Gaetani F (2001) The action of acetyl-l-carnitine on the neurotoxicity evoked by amyloid fragments and peroxide on primary rat cortical neurones

J Cell Biol 161(1):4154

In cosmetic product studies, GHK-Cu was found to tighten loose skin, improve elasticity, density, and firmness, and reduce fine lines, wrinkles, photodamage, and hyperpigmentation