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Subsequently, 100 L of coelenterazine (10 M in PBS pH 7.4) was added, and the luciferase activity was determined using a luminescence microplate reader (Tecan Infinite M-Plex
(PubMed) Noga AA, Vance DE
This is significantly longer than the few seconds of endogenous -MSH, but remains short compared to long-acting analogs such as subcutaneous afamelanotide implant (release over 60 days)
Treating short stature in children due to a deficiency of hGH
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