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In 1999, the MDRD equation for eGFR was published
Suggested Use / Directions: 4 milliliters daily (just under one teaspoon) or as directed by your physician
For electrophysiological recording, cells were seeded onto 12 mm diameter poly-D-lysine coated glass coverslips at a density of in 24-well plates and grown for 48 hours before experiments
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