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The reconstitution process (abbreviated) Swab both vial tops with alcohol Draw calculated water volume into syringe Inject water slowly down vial wall (not onto powder) Wait 30 seconds, then swirl gently (no shaking) Inspect for clarity (clear = good, cloudy = problem) Label vial with concentration and date Refrigerate at 2-8 degrees Celsius Use within 28 days Critical reminders Use bacteriostatic water, never sterile water for multi-dose vials

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Journal of Nutritional Biochemistry, 67(2), 75-82