While this treatment is said to have some benefits, it is not without risks
Foam on the surface (large, persistent bubbles) indicates you either shook the vial or injected water too aggressively
Cellular SOD activity assay NIH/3T3 cells were seeded on 6-well plates (2 10 5 cells per well) and cultured for 24 h
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That said, some study designs still call for a lyophilized vial, particularly when a protocol requires a specific solvent system or a concentration outside the standard spray range