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After washing in ultrapure water for 2 min, the sections were treated with the staining solution (Fluoro-Jade C and 2 g/mL Hoechst diluted in 0.1% acetic acid)

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The study was deemed not to constitute research involving human subjects according to 45 Code of Federal Regulations 46.102(f) and deemed exempt from board oversight and from full review by the RTI International (Research Triangle Park, NC, US) institutional review board

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