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Luciferin-based luminescent assays: Bioluminescent approaches use modified luciferin substrates that release free luciferin upon reaction with GSH

N-terminal acetylation and C-terminal amidation are standard peptide chemistry modifications that preserve the core pharmacophore while improving resistance to enzymatic degradation

Antioxid Redox Signal 15:15831606 Ray PD, Huang B-W, Tsuji Y (2012) Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling

Antioxidants neutralize these harmful molecules, preventing further damage

10.1016/S0969-2126(99)80023-9 Structure

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