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Monitoring overall wellbeing during combined protocols helps identify any interactions or cumulative effects

Prior to transfer, stain-free gels were imaged on a Biorad Chemidoc MP Imaging System (Biorad, Hercules, CA) to confirm equal protein load, and were then wet transferred onto PVDF membranes at 100 V constant for 1 h and equal transfer was routinely confirmed by Ponceau S stain

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For pigmentation patients, particularly those with chronic post-inflammatory hyperpigmentation or skin that has been sensitised by previous aggressive treatments, PN Restore provides a regenerative foundation that stabilises the skins inflammatory response and supports more durable pigmentation treatment outcomes

Climate considerations for Canadian users include storage challenges