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GSH depletion studies: using a laboratory inhibitor such as buthionine sulfoximine (BSO) and confirming the result by direct GSH measurement

Deficient synthesis of glutathione underlies oxidative stress in aging

Nobody has run that study for most of these compounds

Keap1-nrf2 protein-protein interaction inhibitors: design, pharmacological properties and therapeutic potential

GST-tagged proteins are purified from clarified bacterial lysates using immobilized glutathione, and are eluted under mild, non-denaturing conditions to preserve the function of the target protein

24 h after the clamp removal, mice ( n = 8 sham and n = 8 AKI) were injected at the dose of 5 ml/kg via tail vein and were sacrificed 30 min after the injection