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FOXO4-DRI at 25 mM concentration selectively induced p53 nuclear exclusion and apoptosis in the senescent Leydig cells, confirmed by Annexin V-FITC/PI apoptosis assays and immunofluorescent staining of Ser15-phospho-p53 foci
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Compounded formulations combining spironolactone, minoxidil, and tretinoin are increasingly common for female patients
Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems
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For each individual case, the doctor and patient should discuss the patients current health issues, other medications, and family medical history
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