Videos were recorded at 150 frames per segment under consistent illumination and magnification for each experimental condition
After pelleting at 1000 g for 30 s, the worms were incubated in a solution of 20% bleach (sodium hypochlorite), 5% NaOH, and 75% HO with gentle shaking until the adults dissolved, leaving only eggs behind
Another study revealed that 77% of men and 80% of postmenopausal women developed organ dysfunction under choline deprivation, while less than half of premenopausal women did, highlighting the possible protective role of estrogen in choline deficiency
DOI: 10.1111/j.1365-2125.2012.04420.x, Arazi, H., & Eghbali, E
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