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Recently, single-cell (sc) RNA sequence (RNA-seq) analyses of immune cells from brain samples of an AD model (5XFAD) identified microglial clusters named disease-associated microglia (DAM), which appear under neurodegenerative conditions 21

RNA-seq and analysis Total RNA isolation, cDNA library construction, and sequencing were performed at BGI (Beijing Genomics Institute, Shenzhen, Guangdong, China) using RNA-seq technology, as previously described 77,78

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ACOX1 catalyzes the dehydrogenation of acyl-CoA, generating trans-2-enoyl-CoA while transferring electrons directly to O 2 , resulting in the concomitant production of H 2 O 2 [165]