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MOTS-c also influences the folate cycle through AICAR accumulation [2], raising an unresolved question about interaction with methotrexate or other antifolate medications
MS-MS was at 30,000 resolution, AGC target of 300,000, using high-energy collisional dissociation (HCD) at 28V for peptides with 24 charge states and 32V for singly charged ions
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In my own practice, especially if an older person has risk factors for vitamin B12 deficiency, I consider a vitamin B12 level of 200-400 pg/mL borderline, and I usually order a methylmalonic acid level as a follow-up
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